Detection of Human Metadherin by Western Blot. Western blot shows lysates of MDA‑MB‑231 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF membrane was probed with 0.2µg/mL of Sheep Anti-Human Metadherin Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. Specific bands were detected for Metadherin at approximately 80 and 75kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.